Wolf M K, Kardon G B, Adcock L H, Billings-Gagliardi S
Brain Res. 1983 Jul 18;271(1):121-9. doi: 10.1016/0006-8993(83)91371-9.
jp and jpmsd, two allelic mutations in the mouse that sharply reduce the amount of CNS myelin, produce diseases that can be distinguished morphologically only by their severity. This has raised the question of whether the two mutations are truly distinguishable. Since the two mutations have never been maintained on the same genetic background, correct quantitative and morphological comparison have not been possible. We have prepared a B6C3H stock of jp on the same genetic background as the available stock of jpmsd. In this jp stock, behavioral abnormalities, relative proportion of myelinated axons, and major morphological characteristics of the disease in situ are unchanged from the previous jp stock. The jp disease continues to be more severe than that of jpmsd. However, tissue from the new B6C3H stock myelinates better in organotypic culture than previous jp stocks. The increase in myelination is advantageous, not only for accurate comparison of the two alleles but for all culture studies of jp. Strictly comparable strains or stocks should be utilized in any comparative studies of closely related mutations such as jp and jpmsd.
jp和jpmsd是小鼠中的两个等位基因突变，它们会大幅减少中枢神经系统（CNS）的髓磷脂含量，所引发的疾病仅在严重程度上存在形态学差异。这就引发了一个问题，即这两个突变是否真的可以区分。由于这两个突变从未在相同的遗传背景下维持，因此无法进行正确的定量和形态学比较。我们已经培育出了一种与现有jpmsd品系具有相同遗传背景的B6C3H jp品系。在这个jp品系中，行为异常、有髓轴突的相对比例以及疾病在原位的主要形态特征与之前的jp品系没有变化。jp疾病仍然比jpmsd疾病更严重。然而，新的B6C3H品系的组织在器官型培养中比之前的jp品系髓鞘形成得更好。髓鞘形成的增加不仅有利于准确比较这两个等位基因，也有利于所有关于jp的培养研究。在对jp和jpmsd等密切相关突变进行任何比较研究时，都应使用严格可比的品系或株系。
