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Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.

作者信息

Beck H P

出版信息

Cell Tissue Kinet. 1977 May;10(3):265-8. doi: 10.1111/j.1365-2184.1977.tb00294.x.

DOI:10.1111/j.1365-2184.1977.tb00294.x
PMID:68832
Abstract

Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.

摘要

相似文献

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Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.
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Polyamine depletion increases cellular ribonucleotide levels.
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