多胺饥饿延长了多胺依赖性（精氨酸酶缺陷型）中国仓鼠卵巢细胞的S期和G2期。
Polyamine starvation prolongs the S and G2 phases of polyamine-dependent (arginase-deficient) CHO cells.
作者信息
Anehus S, Pohjanpelto P, Baldetorp B, Långström E, Heby O
出版信息
Mol Cell Biol. 1984 May;4(5):915-22. doi: 10.1128/mcb.4.5.915-922.1984.
Abstract
This study analyzes the effects of polyamine starvation on cell cycle traverse of an arginase-deficient CHO cell variant (CHO-A7). These cells grow well in serum-free medium, provided that it contains ornithine or polyamines or both. In the absence of ornithine or polyamines or both, the CHO-A7 cells develop severe polyamine deficiency and, as a consequence, grow more slowly. When grown to a stationary phase in the presence of ornithine or putrescine or both, the CHO-A7 cells became arrested in G0/early G1. However, when starved for ornithine and polyamines, they accumulated in the S and G2 phases. Ornithine and polyamine starvation of CHO-A7 cells causes an increase in ornithine decarboxylase activity. When this increase was prevented by treatment with DL-alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase, growth was further suppressed, and a greater fraction of cells were found in the S and G2 phases of the cell cycle.
摘要
本研究分析了多胺饥饿对精氨酸酶缺陷型CHO细胞变体（CHO-A7）细胞周期进程的影响。这些细胞在无血清培养基中生长良好，前提是该培养基含有鸟氨酸或多胺或两者都有。在缺乏鸟氨酸或多胺或两者都缺乏的情况下，CHO-A7细胞会出现严重的多胺缺乏，结果生长得更慢。当在存在鸟氨酸或腐胺或两者都存在的情况下生长至稳定期时，CHO-A7细胞停滞在G0/早期G1期。然而，当缺乏鸟氨酸和多胺时，它们会在S期和G2期积累。CHO-A7细胞的鸟氨酸和多胺饥饿会导致鸟氨酸脱羧酶活性增加。当用DL-α-二氟甲基鸟氨酸（鸟氨酸脱羧酶的一种酶激活不可逆抑制剂）处理来阻止这种增加时，生长会进一步受到抑制，并且在细胞周期的S期和G2期发现更大比例的细胞。
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本文引用的文献
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Cell cycle parameters of Chinese hamster ovary cells during exponential, polyamine-limited growth.中国仓鼠卵巢细胞在指数生长期、多胺限制生长期间的细胞周期参数。
Mol Cell Biol. 1981 Jul;1(7):594-9. doi: 10.1128/mcb.1.7.594-599.1981.
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Polyamine starvation causes disappearance of actin filaments and microtubules in polyamine-auxotrophic CHO cells.多胺饥饿导致多胺营养缺陷型中国仓鼠卵巢细胞中的肌动蛋白丝和微管消失。
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Polyamine dependence of Chinese hamster ovary cells in serum-free culture is due to deficient arginase activity.中国仓鼠卵巢细胞在无血清培养中对多胺的依赖性是由于精氨酸酶活性不足。
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10
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