嗜盐栖热菌的噬菌体：病毒体DNA和蛋白质

Bacteriophages of Halobacterium halobium: virion DNAs and proteins.

作者信息

Rohrmann G F, Cheney R, Pauling C

出版信息

Can J Microbiol. 1983 May;29(5):627-9. doi: 10.1139/m83-100.

Abstract

The DNAs from bacteriophages Hh-1 and Hh-3 that infect Halobacterium halobium were characterized. Both phages contain linear double-stranded DNA and show no relatedness based on restriction endonuclease fragment patterns. Hh-1 DNA has 67.05% guanine plus cytosine (G+C) and a molecular weight of 24.6 X 10(6), whereas Hh-3 DNA has 62.15% G+C and a molecular weight of 19.4 X 10(6). Polyacrylamide gel electrophoresis indicated that the major proteins of the two phages are of different molecular weights.

摘要

对感染嗜盐栖热菌的噬菌体Hh-1和Hh-3的DNA进行了表征。两种噬菌体均含有线性双链DNA，根据限制性内切酶片段模式显示无相关性。Hh-1 DNA的鸟嘌呤加胞嘧啶（G+C）含量为67.05%，分子量为24.6×10⁶，而Hh-3 DNA的G+C含量为62.15%，分子量为19.4×10⁶。聚丙烯酰胺凝胶电泳表明，两种噬菌体的主要蛋白质分子量不同。