• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肺炎链球菌的温和噬菌体，其DNA的5'端含有共价连接的蛋白质。

Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA.

作者信息

Romero A, Lopez R, Lurz R, Garcia P

机构信息

Centro de Investigaciones Biológicas, Madrid, Spain.

出版信息

J Virol. 1990 Oct;64(10):5149-55. doi: 10.1128/JVI.64.10.5149-5155.1990.

DOI:10.1128/JVI.64.10.5149-5155.1990
PMID:2398537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248007/
Abstract

We have characterized three temperate bacteriophages of pneumococcus (HB-3, HB-623, and HB-746). Although all the phages belong to the same family, the polypeptide composition of the virions and the DNA restriction endonuclease analysis of their DNAs revealed differences among the three phages. The genomes of these bacteriophages have been isolated as DNA-protein complexes. The protein is specifically associated with the two 5' termini of the DNA as shown by experiments carried out with exonucleases. The protein bound to the DNA in the three phages studied, iodinated in vitro with 125I, has a molecular weight of 23,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Treatment of the complexes with chaotropic agents suggested that the protein is covalently bound to the 5' termini of the DNA. Comparative pulsed-field gel electrophoresis analysis and Southern hybridization of the SmaI restriction fragments of DNAs from one lysogenic bacteria and its parental strain revealed that the prophage genome was integrated in the host chromosome.

摘要

我们已经鉴定了肺炎球菌的三种温和噬菌体（HB-3、HB-623和HB-746）。尽管所有噬菌体都属于同一科，但病毒粒子的多肽组成及其DNA的限制性内切酶分析显示这三种噬菌体之间存在差异。这些噬菌体的基因组已作为DNA-蛋白质复合物被分离出来。如用核酸外切酶进行的实验所示，该蛋白质与DNA的两个5'末端特异性结合。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，在体外经125I碘化的、与所研究的三种噬菌体中的DNA结合的蛋白质，其分子量为23,000。用离液剂处理这些复合物表明该蛋白质与DNA的5'末端共价结合。对来自一株溶原性细菌及其亲代菌株的DNA的SmaI限制性片段进行比较脉冲场凝胶电泳分析和Southern杂交，结果显示原噬菌体基因组整合到了宿主染色体中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/01fb61698603/jvirol00065-0562-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/5010b2027811/jvirol00065-0558-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/aa2f3fc7aa24/jvirol00065-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/00049c98d26f/jvirol00065-0560-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/09c9bcd7e57c/jvirol00065-0560-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/d7bf0401dca5/jvirol00065-0561-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/01fb61698603/jvirol00065-0562-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/5010b2027811/jvirol00065-0558-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/aa2f3fc7aa24/jvirol00065-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/00049c98d26f/jvirol00065-0560-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/09c9bcd7e57c/jvirol00065-0560-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/d7bf0401dca5/jvirol00065-0561-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92d/248007/01fb61698603/jvirol00065-0562-a.jpg

相似文献

1
Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA.肺炎链球菌的温和噬菌体，其DNA的5'端含有共价连接的蛋白质。
J Virol. 1990 Oct;64(10):5149-55. doi: 10.1128/JVI.64.10.5149-5155.1990.
2
Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5' ends.肺炎链球菌噬菌体DNA的限制性切割图谱，这些噬菌体的5'端含有共价结合的蛋白质。
Mol Gen Genet. 1984;197(1):67-74. doi: 10.1007/BF00327924.
3
EJ-1, a temperate bacteriophage of Streptococcus pneumoniae with a Myoviridae morphotype.EJ-1，一种具有肌尾噬菌体形态型的肺炎链球菌温和噬菌体。
J Bacteriol. 1992 Sep;174(17):5516-25. doi: 10.1128/jb.174.17.5516-5525.1992.
4
Peculiarities of the DNA of MM1, a temperate phage of Streptococcus pneumoniae.肺炎链球菌温和噬菌体MM1的DNA特性
Int Microbiol. 2004 Jun;7(2):133-7.
5
Prophage distribution in coryneform bacteria.棒状杆菌中的原噬菌体分布
Res Microbiol. 1995 Jul-Aug;146(6):493-505. doi: 10.1016/0923-2508(96)80295-6.
6
Characterization and comparison of virulent bacteriophages of Streptococcus thermophilus isolated from yogurt.从酸奶中分离出的嗜热链球菌烈性噬菌体的特性及比较
Biochimie. 1990 Dec;72(12):855-62. doi: 10.1016/0300-9084(90)90002-x.
7
Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5' termini of its DNA.肺炎球菌噬菌体Cp-1含有一种与DNA 5'末端结合的蛋白质。
Virology. 1983 Jul 15;128(1):92-104. doi: 10.1016/0042-6822(83)90321-5.
8
The insertion site of the temperate phage HB-746 is located near the phage remnant in the pneumococcal host chromosome.温和噬菌体HB-746的插入位点位于肺炎球菌宿主染色体中噬菌体残余物附近。
J Virol. 1992 May;66(5):2860-4. doi: 10.1128/JVI.66.5.2860-2864.1992.
9
[Wide distribution of transposable phages in natural Pseudomonas aeruginosa populations].[转座噬菌体在天然铜绿假单胞菌群体中的广泛分布]
Genetika. 1984 Oct;20(10):1612-9.
10
Electron microscopic heteroduplex study and restriction endonuclease cleavage analysis of the DNA genomes of three lactic streptococcal bacteriophages.三种乳酸链球菌噬菌体DNA基因组的电子显微镜异源双链研究及限制性内切酶切割分析
Appl Environ Microbiol. 1986 Mar;51(3):566-71. doi: 10.1128/aem.51.3.566-571.1986.

引用本文的文献

1
A genomic approach to understand interactions between Streptococcus pneumoniae and its bacteriophages.一种用于理解肺炎链球菌与其噬菌体之间相互作用的基因组学方法。
BMC Genomics. 2015 Nov 18;16:972. doi: 10.1186/s12864-015-2134-8.
2
Genome annotation and intraviral interactome for the Streptococcus pneumoniae virulent phage Dp-1.肺炎链球菌烈性噬菌体 Dp-1 的基因组注释及病毒内相互作用组
J Bacteriol. 2011 Jan;193(2):551-62. doi: 10.1128/JB.01117-10. Epub 2010 Nov 19.
3
Prophage genomics.前噬菌体基因组学

本文引用的文献

1
The Use of Benzoylvinyl-Diacetone-Alkamine Lactate.苯甲酰乙烯基-二丙酮-烷胺乳酸酯的用途。
Cal State J Med. 1905 May;3(5):142-3.
2
Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of maize.玉米 S 细胞质中 S-2 线粒体 DNA 的核苷酸序列。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4055-9. doi: 10.1073/pnas.80.13.4055.
3
Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize.与雄性不育玉米“S”型细胞质中线粒体相关的独特DNA。
Microbiol Mol Biol Rev. 2003 Jun;67(2):238-76, table of contents. doi: 10.1128/MMBR.67.2.238-276.2003.
4
Genome organization and molecular analysis of the temperate bacteriophage MM1 of Streptococcus pneumoniae.肺炎链球菌温和噬菌体MM1的基因组组织与分子分析
J Bacteriol. 2003 Apr;185(7):2362-8. doi: 10.1128/JB.185.7.2362-2368.2003.
5
MM1, a temperate bacteriophage of the type 23F Spanish/USA multiresistant epidemic clone of Streptococcus pneumoniae: structural analysis of the site-specific integration system.MM1，一种肺炎链球菌23F型西班牙/美国多重耐药流行克隆株的温和噬菌体：位点特异性整合系统的结构分析
J Virol. 2000 Sep;74(17):7803-13. doi: 10.1128/jvi.74.17.7803-7813.2000.
6
Tailed bacteriophages: the order caudovirales.有尾噬菌体：长尾噬菌体目
Adv Virus Res. 1998;51:135-201. doi: 10.1016/s0065-3527(08)60785-x.
7
The adenovirus terminal protein influences binding of replication proteins and changes the origin structure.腺病毒末端蛋白影响复制蛋白的结合并改变起始结构。
Nucleic Acids Res. 1993 May 25;21(10):2293-300. doi: 10.1093/nar/21.10.2293.
8
Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris.野油菜黄单胞菌野油菜致病变种的一种广义转导噬菌体的分离与特性分析
J Bacteriol. 1994 Jun;176(11):3354-9. doi: 10.1128/jb.176.11.3354-3359.1994.
9
The insertion site of the temperate phage HB-746 is located near the phage remnant in the pneumococcal host chromosome.温和噬菌体HB-746的插入位点位于肺炎球菌宿主染色体中噬菌体残余物附近。
J Virol. 1992 May;66(5):2860-4. doi: 10.1128/JVI.66.5.2860-2864.1992.
10
EJ-1, a temperate bacteriophage of Streptococcus pneumoniae with a Myoviridae morphotype.EJ-1，一种具有肌尾噬菌体形态型的肺炎链球菌温和噬菌体。
J Bacteriol. 1992 Sep;174(17):5516-25. doi: 10.1128/jb.174.17.5516-5525.1992.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2904-8. doi: 10.1073/pnas.74.7.2904.
4
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
5
Inhibition of transformation in Streptococcus pneumoniae by lysogeny.溶原性对肺炎链球菌转化的抑制作用。
J Bacteriol. 1980 Mar;141(3):1298-304. doi: 10.1128/jb.141.3.1298-1304.1980.
6
The maintenance of the P1 plasmid prophage.
Plasmid. 1981 Jan;5(1):20-31. doi: 10.1016/0147-619x(81)90075-5.
7
New species definitions in phages of gram-positive cocci.革兰氏阳性球菌噬菌体的新物种定义。
Intervirology. 1984;22(4):181-90. doi: 10.1159/000149550.
8
A new mechanism for the initiation of replication of phi 29 and adenovirus DNA: priming by the terminal protein.
Curr Top Microbiol Immunol. 1984;109:89-106. doi: 10.1007/978-3-642-69460-8_4.
9
Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5' termini of its DNA.肺炎球菌噬菌体Cp-1含有一种与DNA 5'末端结合的蛋白质。
Virology. 1983 Jul 15;128(1):92-104. doi: 10.1016/0042-6822(83)90321-5.
10
Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replication.一种启动DNA复制所需的腺病毒编码DNA聚合酶的纯化。
Cell. 1982 Dec;31(3 Pt 2):613-23. doi: 10.1016/0092-8674(82)90317-8.