Moazamie N, Ackermann H W, Murthy M R
Can J Microbiol. 1979 Sep;25(9):1063-72. doi: 10.1139/m79-163.
Salmonella newport phages 16--19 and 7--11 have very long heads and are members of two rare and so far little-known phage groups. Both produce various morphological aberrations. Preparations of phage 7--11 contain numerous polyheads and about 0.4% short heads belonging to nine size classes. In addition, one giant phage particle was observed. The head of phage 7--11 seems to be an icosahedron which became elongated by adding successive rows of subunits. Phages 16--19 and 7--11 have buoyant densities in CsCl of 1.43 and 1.48 g/mL and particle weights of 103 and 204 x 10(6) respectively. Both viruses contain double-stranded DNA, internal proteins, and sugars. Phage 16--19 contains 46.5% DNA of 35 x 10(6) molecular weight, and glucose. Phage 7--11 contains 47.5% DNA of 108 x 10(6) molecular weight, and mannose. Base compositions of phage and S. newport DNAs were determined from buoyant densities, melting point, and acid hydrolysis. Phage 16--19 contains 5.4% 5-methylcytosine.
新港沙门氏菌噬菌体16 - 19和7 - 11具有非常长的头部，属于两个罕见且迄今鲜为人知的噬菌体群。两者都会产生各种形态畸变。噬菌体7 - 11的制剂含有大量多聚头部以及约0.4%属于九个大小类别的短头部。此外，还观察到一个巨型噬菌体颗粒。噬菌体7 - 11的头部似乎是一个二十面体，通过添加连续的亚基排而变长。噬菌体16 - 19和7 - 11在CsCl中的浮力密度分别为1.43和1.48 g/mL，颗粒重量分别为103和204×10⁶。两种病毒都含有双链DNA、内部蛋白质和糖类。噬菌体16 - 19含有分子量为35×10⁶的46.5%的DNA以及葡萄糖。噬菌体7 - 11含有分子量为108×10⁶的47.5%的DNA以及甘露糖。根据浮力密度、熔点和酸水解确定了噬菌体和新港沙门氏菌DNA的碱基组成。噬菌体16 - 19含有5.4%的5 - 甲基胞嘧啶。
