Muhrer K H, Link K H
Cancer Detect Prev. 1983;6(1-2):101-6.
Specificity of cellular immune reactivity was tested in 182 cancer patients, 89 patients with inflammatory diseases, and 61 healthy controls. The in vitro method of electrophoretic mobility testing permitted evaluation of cellular immune reactivity. Soluble potassium chloride extracts from various human carcinomas were used as antigens. Out of 182 cancer patients 164 (90.1%) showed positive test results using extracts from tumors of the same type as the patient's tumor. Using the same tumor extracts in 61 healthy control persons, a total of 182 tests showed negative results in 156 cases (85.7%). Lymphocytic reactions to various tumor extracts varied in a typical pattern: The most pronounced migration inhibition was observed when tumor patients were tested against extracts of carcinomas of the same histogenesis. Tumor extracts deriving from different organs had a weaker or negative reaction. For each tumor a typical lymphocytic reaction pattern was noted. The method seems also to detect precancerous lesions; out of 19 patients with colorectal adenomas, 12 showed positive reactions to extracts from colorectal cancer. Inflammatory diseases of the various organs reduced the reliability of the method. Demonstrated specificity of sensitized lymphocytes could prove to be valuable aid in immunological tumor recognition.
在182例癌症患者、89例炎症性疾病患者和61名健康对照者中测试了细胞免疫反应性的特异性。体外电泳迁移率测试方法可用于评估细胞免疫反应性。使用从各种人类癌组织中提取的可溶性氯化钾提取物作为抗原。182例癌症患者中，164例（90.1%）使用与患者肿瘤相同类型的肿瘤提取物进行测试时显示阳性结果。在61名健康对照者中使用相同的肿瘤提取物进行测试，总共182次测试中有156例（85.7%）显示阴性结果。淋巴细胞对各种肿瘤提取物的反应呈现出典型的模式：当肿瘤患者用来自相同组织发生的癌组织提取物进行测试时，观察到最明显的迁移抑制。来自不同器官的肿瘤提取物反应较弱或呈阴性。对于每种肿瘤，都观察到了典型的淋巴细胞反应模式。该方法似乎还能检测癌前病变；在19例大肠腺瘤患者中，12例对结直肠癌提取物呈阳性反应。各器官的炎症性疾病降低了该方法的可靠性。所证明的致敏淋巴细胞的特异性可能在免疫肿瘤识别中成为有价值的辅助手段。
