Wargovich M J, Eng V W, Newmark H L, Bruce W R
Carcinogenesis. 1983 Sep;4(9):1205-7. doi: 10.1093/carcin/4.9.1205.
The effect of a deoxycholic acid instillation on colonic epithelium was investigated and compared with the effect of an intervention scheme in which deoxycholic acid (DCA) treated mice received oral supplements of calcium lactate. The morphology of colonic tissue exposed to DCA was markedly affected when compared to that of untreated controls. Inflammation, edema, and necrosis preceded elevated numbers of mitotic figures appearing 24 h to 48 h after DCA treatment. Proliferative activity as measured by tritiated thymidine uptake and autoradiography was increased 2.5-fold in colonic crypts of DCA treated mice. In contrast mice receiving multiple oral supplements of calcium lactate showed minimal tissue necrosis due to DCA administration and the frequency of mitotic events and cellular proliferation activity remained similar to levels seen in untreated controls. Sequestration of bile acids by calcium salts may provide a scheme for inhibiting the untoward effects of bile acids on the colonic epithelium and a mechanism for blocking the reported co-carcinogenic activity of these agents.
研究了脱氧胆酸灌注对结肠上皮的影响，并将其与一种干预方案的效果进行了比较，在该方案中，用脱氧胆酸（DCA）处理的小鼠接受乳酸钙口服补充剂。与未处理的对照组相比，暴露于DCA的结肠组织形态受到显著影响。在DCA处理后24至48小时，炎症、水肿和坏死先于有丝分裂图像数量的增加出现。通过氚标记胸腺嘧啶核苷摄取和放射自显影测量的增殖活性在DCA处理的小鼠结肠隐窝中增加了2.5倍。相比之下，接受多次乳酸钙口服补充剂的小鼠由于给予DCA而显示出最小的组织坏死，有丝分裂事件的频率和细胞增殖活性仍与未处理对照组所见水平相似。钙盐对胆汁酸的螯合作用可能提供一种抑制胆汁酸对结肠上皮不良影响的方案，以及一种阻断这些药物所报道的促癌活性的机制。
