Dasgupta M K, Davis P, Johny K V, Higgins M R, Dossetor J B
Clin Invest Med. 1983;6(2):73-7.
Although the presence of free DNA and antibodies to DNA in the sera of patients on maintenance hemodialysis has been reported, we found only a low incidence of antibodies to single-stranded DNA (ss-DNA) and native DNA (n-DNA) using an improved detection technique. In our search for evidence of the possible formation of DNA-anti-DNA circulating immune complexes (CIC), antibodies to n-DNA were detected in the sera of only 2 of 53 dialysed and 1 of 25 non-dialysed renal patients. CICs were detected in the sera of 17 of 53 dialysed (28% by Raji-RIA, 6% by Clq-BA) and 6 of 25 non-dialysed (20% by Raji-RIA and 24% by Clq-BA) renal patients: the difference is not significant (P greater than 0.25). DNase digestion of the dialysed sera that were positive for CIC by Raji-RIA produced no significant change in the results on re-assay (P greater than 0.50) in contrast with a significant decrease in CIC after DNase digestion of selected SLE sera and of solutions containing DNA-anti-DNA complexes formed in vitro (P less than 0.05). The formation of circulating DNA-anti-DNA complexes appears not to be the cause of the low incidence of antibodies to n-DNA in the sera of dialysed patients.
尽管已有报道维持性血液透析患者血清中存在游离DNA及抗DNA抗体，但我们采用改良检测技术发现，单链DNA（ss-DNA）和天然DNA（n-DNA）抗体的发生率很低。在我们寻找DNA-抗DNA循环免疫复合物（CIC）可能形成证据的过程中，53例透析肾患者血清中仅2例、25例未透析肾患者血清中仅1例检测到抗n-DNA抗体。53例透析肾患者血清中有17例（Raji-RIA法检测为28%，Clq-BA法检测为6%）、25例未透析肾患者血清中有6例（Raji-RIA法检测为20%，Clq-BA法检测为24%）检测到CIC：差异无统计学意义（P>0.25）。与选定的系统性红斑狼疮（SLE）患者血清及体外形成的含DNA-抗DNA复合物的溶液经DNase消化后CIC显著降低（P<0.05）相反，Raji-RIA法检测CIC呈阳性的透析患者血清经DNase消化后重新检测结果无显著变化（P>0.50）。循环DNA-抗DNA复合物的形成似乎不是透析患者血清中抗n-DNA抗体发生率低的原因。
