Clarke C W
Clin Allergy. 1977 Jan;7(1):41-7. doi: 10.1111/j.1365-2222.1977.tb01423.x.
Three antigenic preparations were obtained from a non-capsulated strain of Haemophilus influenzae by ultrasonic disintegration, hot phenol extraction and from a fluid culture. They were designated H. influenzae cytoplasmic antigen (H(1-5); H. influenzae cell wall antigen (HCW); and H. influenzae culture filtrate antigen (HCF). Studies showed that H(1-5) antigen contained heat stable and heat labile components. The heat stable fraction stained positively for polysaccharide, had a positive limulus lysate test and there was immunological cross-reactivity between this and heat stable fractions of HCW and HCF. Limulus lysate assay indicated the presence of endotoxin in HCW and HCF preparations. Heat stable as well as heat labile antigens of H. influenzae should be given consideration in future studies regarding the pathogenicity of this organism in the lower respiratory tree. The specificity of the heat stable antigen of H. influenzae needs to be determined.
通过超声破碎、热酚提取以及从液体培养物中，从一株无荚膜流感嗜血杆菌中获得了三种抗原制剂。它们分别被命名为流感嗜血杆菌细胞质抗原（H(1-5)）、流感嗜血杆菌细胞壁抗原（HCW）和流感嗜血杆菌培养滤液抗原（HCF）。研究表明，H(1-5)抗原包含热稳定和热不稳定成分。热稳定部分对多糖染色呈阳性，鲎试剂检测呈阳性，并且该部分与HCW和HCF的热稳定部分之间存在免疫交叉反应。鲎试剂检测表明HCW和HCF制剂中存在内毒素。在未来关于该生物体在下呼吸道致病性的研究中，应考虑流感嗜血杆菌的热稳定抗原以及热不稳定抗原。流感嗜血杆菌热稳定抗原的特异性需要确定。
