Clarke C W
Br J Dis Chest. 1979 Oct;73(4):373-81.
The frequency of precipitating antibody to heat-labile (H(1--2) and heat-stable (HCW and HCF) antigens of Haemophilus influenzae was determined in patients with asthma, chronic bronchitis, cystic fibrosis and bronchiectasis and compared with that in a control group. This showed that the immune response of asthmatic patients to heat-stable antigens was different from that to the heat-labile antigens. Exposure to antigens of H. influenzae is common in all the disease groups. Skin test reactions having the time course and macroscopic appearance of Type I (immediate) and Type III (late) were obtained after prick and intracutaneous skin testing with HCW antigen in varying concentrations in a group of patients with asthma, chronic bronchitis or cystic fibrosis and in a control group. It is suggested that IgE and short-term sensitizing IgG antibodies may be responsible for the immediate reactions while activation of the alternative pathway of complement by endotoxin contained in HCW could be responsible for the late reactions. HCW antigens were shown to release histamine from non-sensitized human leucocytes; HCW and HCF antigens were shown to release histamine from non-sensitized human lung. None of the antigens tested had an effect on beta-receptors in tracheal preparations. It is proposed that these reactions may contribute to the pathogenicity of H. influenzae in the lower respiratory tract.
测定了哮喘、慢性支气管炎、囊性纤维化和支气管扩张患者针对流感嗜血杆菌不耐热（H(1--2)）和耐热（HCW和HCF）抗原的沉淀抗体频率，并与对照组进行了比较。结果表明，哮喘患者对耐热抗原的免疫反应与对不耐热抗原的免疫反应不同。在所有疾病组中，接触流感嗜血杆菌抗原都很常见。在一组哮喘、慢性支气管炎或囊性纤维化患者以及对照组中，用不同浓度的HCW抗原进行针刺和皮内皮肤试验后，获得了具有I型（即刻）和III型（迟发）时间进程和宏观外观的皮肤试验反应。提示IgE和短期致敏IgG抗体可能是即刻反应的原因，而HCW中所含内毒素激活补体替代途径可能是迟发反应的原因。已证明HCW抗原可从非致敏人白细胞中释放组胺；已证明HCW和HCF抗原可从非致敏人肺中释放组胺。所测试的抗原均对气管制剂中的β受体无影响。有人提出，这些反应可能有助于流感嗜血杆菌在下呼吸道的致病性。
