Moore C, Blank M L, Lee T C, Benjamin B, Piantadosi C, Snyder F
Chem Phys Lipids. 1978 Jun;21(3):175-8. doi: 10.1016/0009-3084(78)90064-6.
In previous studies on the modification of polar head groups of membrane phospholipids with the unnatural base analog, N-isopropylethanolamine, we reported an unidentified phospholipid in addition to phosphatidyl-N-isopropylethanolamine in the various membrane fractions of rat liver. The structure of this phospholipid has now been identified as phosphatidyl-N-methyl-N-isopropylethanolamine by nuclear magnetic resonance spectroscopy, and by chromatographic and enzymic analysis. In addition, we found that when rats were injected intraperitoneally with the N-methyl-N-isopropylethanolamine, 19% of the liver microsomal phospholipid was phosphatidyl-N-methyl-N-isopropylethanolamine.
在之前关于用非天然碱基类似物N-异丙基乙醇胺修饰膜磷脂极性头部基团的研究中，我们报道在大鼠肝脏的各种膜组分中，除了磷脂酰-N-异丙基乙醇胺外，还存在一种未鉴定的磷脂。现在，通过核磁共振光谱、色谱分析和酶分析，已确定这种磷脂的结构为磷脂酰-N-甲基-N-异丙基乙醇胺。此外，我们发现，当给大鼠腹腔注射N-甲基-N-异丙基乙醇胺时，肝脏微粒体磷脂中有19%是磷脂酰-N-甲基-N-异丙基乙醇胺。
