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常规卡介苗制剂与实验性卡介苗制剂的摄氧率及三磷酸腺苷含量比较。

A comparison of the rate of oxygen uptake and the adenosine triphosphate content of routine and experimental BCG preparations.

作者信息

Borremans M, Weckx M, Verhofstadt R

出版信息

J Biol Stand. 1983 Jul;11(3):205-12. doi: 10.1016/s0092-1157(83)80007-9.

DOI:10.1016/s0092-1157(83)80007-9
PMID:6885826
Abstract

The ATP content and oxygen uptake rate, two parameters of viability of a BCG suspension, are compared. The lack of correlation between measurements made on fresh routine preparations indicates that the ATP measurements is affected by the degree of dispersion of the preparation. Experimental preparations made under standard conditions (constant semi-dry weight and stepwise dispersive grinding) from cultures of the same age as the fresh routine cultures (14 days) had a smaller ATP content, which correlated well with the respiration rate. In experimental preparations from seven-day cultures this correlation was significant. Between days 7 and 14 the ATP content declined much more rapidly than the respiration rate.

摘要

比较了卡介苗悬浮液活力的两个参数——三磷酸腺苷(ATP)含量和氧摄取率。对新鲜常规制剂进行的测量之间缺乏相关性,这表明ATP测量受制剂分散程度的影响。在标准条件下(恒定半干重和逐步分散研磨)从与新鲜常规培养物相同培养时间(14天)的培养物中制备的实验制剂,其ATP含量较低,且与呼吸速率相关性良好。在来自7天培养物的实验制剂中,这种相关性很显著。在第7天到第14天之间,ATP含量下降的速度比呼吸速率快得多。

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