Patterson D, Vannais D B, Laas W
J Cell Physiol. 1983 Sep;116(3):257-64. doi: 10.1002/jcp.1041160302.
The isolation and characterization of a new pyrimidine requiring mutant of Chinese hamster ovary cells (CHO-Kl, pro-) is described. This mutant (P192) shows an absolute requirement for uridine for growth but is fully capable of pyrimidine nucleotide synthesis. P192 cells accumulate exceedingly large amounts of a compound identified by a variety of chromatographic, chemical, and labelling criteria as UDP glucuronic acid (UDPglcUA). This compound comprises approximately 60% of the acid-soluble nucleotides of P192 cells. Possible genetic mechanisms leading to the phenotype of P192 are examined, and the role of UDPglcUA in cellular metabolism is discussed.
本文描述了中国仓鼠卵巢细胞（CHO-K1，脯氨酸缺陷型）一个新的嘧啶需求突变体的分离与特性鉴定。该突变体（P192）生长绝对需要尿苷，但完全能够进行嘧啶核苷酸合成。P192细胞积累了大量经多种色谱、化学和标记标准鉴定为尿苷二磷酸葡糖醛酸（UDPglcUA）的化合物。该化合物约占P192细胞酸溶性核苷酸的60%。研究了导致P192表型的可能遗传机制，并讨论了UDPglcUA在细胞代谢中的作用。
