普萘洛尔代谢产物对人血浆中普萘洛尔荧光测定法的影响。

The contribution of propranolol metabolites to the fluorometric assay of propranolol in human plasma.

Pritchard J F, Schneck D W, Racz W J, Hayes A H

Clin Biochem. 1978 Jun;11(3):121-5. doi: 10.1016/s0009-9120(78)90154-6.

Abstract

Studies were undertaken to determine the fluorescent properties of several propranolol metabolites under the conditions of the fluorometric propranolol assay. Of the metabolites studied, propranolol glycol and N-desisopropylpropranolol had significant molar fluorescent coefficients relative to propranolol (72 and 79% respectively). N-desisopropylpropranolol was extracted with the same efficiency as propranolol (greater than 90%) wheras the glycol metabolite had only 34% extraction efficiency. Addition of each metabolite to samples of human plasma containing propranolol produced the predicted increase in fluorescent intensity. 2. Gas chromatographic analysis of plasma collected from 22 hypertensive patients chronically receiving oral propranolol revealed low concentrations of propranolol glycol and N-desisopropylpropranolol relative to propranolol. The results of these studies indicate that fluorescent metabolites of propranolol are not present in sufficient concentration to significantly interfere with the fluorometric assay of propranolol.

摘要

开展了多项研究，以确定在荧光法测定普萘洛尔的条件下几种普萘洛尔代谢物的荧光特性。在所研究的代谢物中，普萘洛尔二醇和N-去异丙基普萘洛尔相对于普萘洛尔具有显著的摩尔荧光系数（分别为72%和79%）。N-去异丙基普萘洛尔的提取效率与普萘洛尔相同（大于90%），而二醇代谢物的提取效率仅为34%。将每种代谢物添加到含有普萘洛尔的人血浆样本中，会使荧光强度出现预期的增加。2. 对22名长期口服普萘洛尔的高血压患者采集的血浆进行气相色谱分析，结果显示相对于普萘洛尔，普萘洛尔二醇和N-去异丙基普萘洛尔的浓度较低。这些研究结果表明，普萘洛尔的荧光代谢物浓度不足以显著干扰普萘洛尔的荧光测定法。