通过直接液体引入对生物源儿茶酚胺离子对进行质谱分析。

Mass spectrometry of biogenic catecholamine ion pairs by direct liquid introduction.

作者信息

Milon H, Bur H

出版信息

J Chromatogr. 1983 Apr 22;271(1):83-92. doi: 10.1016/s0021-9673(00)80201-0.

Abstract

The mass spectrometry of ion pairs of dopamine, noradrenaline and 3,4-dihydroxyphenylacetic acid with n-octylsulphonate has been investigated by direct liquid introduction in an acidic buffer currently used for their high-performance liquid chromatographic separation. In order to follow the fate of the ion pairs, they were first studied by desorption/chemical ionization as dichloromethane extracts or in the buffer. The results show that characteristic spectra of the solutes can be obtained by direct liquid introduction provided that the interface between the chromatographic system and the mass spectrometer permits the desolvation of the ion pairs from the cluster of solvent molecules.

摘要

采用直接液体进样法，在目前用于多巴胺、去甲肾上腺素和3,4-二羟基苯乙酸高效液相色谱分离的酸性缓冲液中，研究了它们与正辛基磺酸盐离子对的质谱。为了追踪离子对的去向，首先将它们作为二氯甲烷提取物或在缓冲液中通过解吸/化学电离进行研究。结果表明，只要色谱系统与质谱仪之间的接口能够使离子对从溶剂分子簇中去溶剂化，通过直接液体进样就能获得溶质的特征光谱。