使用微径高效液相色谱法和电化学检测法测定生物胺的飞克检测限。
Femtogram detection limits for biogenic amines using microbore HPLC with electrochemical detection.
作者信息
Caliguri E J, Mefford I N
出版信息
Brain Res. 1984 Mar 26;296(1):156-9. doi: 10.1016/0006-8993(84)90523-7.
PMID:6201234
Abstract
Detection limits for biogenic amines, serotonin (5-HT), 5-hydroxyindoleacetic acid, 5-hydroxytryptophan, norepinephrine, epinephrine, dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), of 50-200 fg are reported. This was accomplished using microbore HPLC with electrochemical detection. Reproducibility at the 1-5 pg level of 1.6-8.1% was obtained. The utility of this approach is demonstrated for analysis of brain tissues of 1 microgram or less.
摘要
据报道，生物胺、血清素（5-羟色胺，5-HT）、5-羟吲哚乙酸、5-羟色氨酸、去甲肾上腺素、肾上腺素、多巴胺和3,4-二羟基苯乙酸（DOPAC）的检测限为50-200飞克。这是通过采用电化学检测的微径高效液相色谱法实现的。在1-5皮克水平上获得了1.6-8.1%的重现性。该方法的实用性在分析1微克或更少的脑组织中得到了证明。
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