Tobler P H, Tschopp F A, Dambacher M A, Born W, Fischer J A
J Clin Endocrinol Metab. 1983 Oct;57(4):749-54. doi: 10.1210/jcem-57-4-749.
Different immunoreactive calcitonin (CT) forms were identified by a reversed phase high performance liquid chromatography gradient system in plasma and urine of normal subjects. The separated CT components were characterized by chemical and enzymatic methods and found to be identical in normal subjects and medullary thyroid carcinoma patients. Of seven major CT forms we identified monomeric human CT-(1-32), its sulfoxide form, and dimeric CT in plasma. The monomeric, but not the dimeric form, was also detected in urine. A predominant CT component in plasma with a molecular weight of about 12,000 may correspond to a biosynthetic precursor of the hormone.
通过反相高效液相色谱梯度系统在正常受试者的血浆和尿液中鉴定出不同的免疫反应性降钙素（CT）形式。分离出的CT成分通过化学和酶学方法进行表征，发现其在正常受试者和甲状腺髓样癌患者中是相同的。在我们鉴定出的七种主要CT形式中，血浆中有单体人CT-（1-32）、其亚砜形式和二聚体CT。尿液中也检测到了单体形式，但未检测到二聚体形式。血浆中一种主要的分子量约为12,000的CT成分可能对应于该激素的生物合成前体。
