Oohata A A
J Embryol Exp Morphol. 1983 Apr;74:311-9.
A simple and reliable method of separating and quantifying acid phosphatase activities in D. discoideum is described, and one of the electrophoretically distinguishable forms of the enzyme is shown to be specific for prestalk cells. Accumulation of this product commences some 2-3 h later in development than the prespore specific enzyme UDP-galactose polysaccharide transferase. This finding is discussed in terms of recent observation on the control of prestalk cell differentiation in this organism.
本文描述了一种简单可靠的方法，用于分离和定量盘基网柄菌中的酸性磷酸酶活性，并且该酶的一种可通过电泳区分的形式被证明对柄细胞前体具有特异性。与孢子前体特异性酶UDP-半乳糖多糖转移酶相比，这种产物的积累在发育过程中开始的时间要晚约2-3小时。根据最近对该生物体中柄细胞前体分化控制的观察结果对这一发现进行了讨论。
