Oohata A A
Cell Differ. 1986 Dec;19(4):271-9. doi: 10.1016/0045-6039(86)90104-1.
The electrophoretic pattern of intracellular and secreted acid phosphatases (AcPases) in Dictyostelium discoideum was examined during incubation of the cells as a submerged monolayer. Three distinct forms of the enzyme were observed in the cell during differentiation; one was detected throughout development (AcPase 1), whereas the others including AcPase 2 were stage-specific. AcPase 1 was released in the medium predominantly in early development and AcPase 2, a prestalk specific form, was secreted during stalk formation. When cells were incubated under conditions where stalk cells did not form, only AcPase 1 was recognized both in the cell and in the medium.
在盘基网柄菌作为浸没单层细胞培养期间，检测了其细胞内和分泌型酸性磷酸酶（AcPases）的电泳图谱。在细胞分化过程中观察到该酶有三种不同形式；一种在整个发育过程中都能检测到（酸性磷酸酶1），而其他形式包括酸性磷酸酶2是阶段特异性的。酸性磷酸酶1主要在发育早期释放到培养基中，而酸性磷酸酶2是一种前柄特异性形式，在柄形成过程中分泌。当细胞在不形成柄细胞的条件下培养时，在细胞内和培养基中都只能识别到酸性磷酸酶1。
