Litin B S, Swan K R, Koldovský O
J Pediatr Gastroenterol Nutr. 1983;2(1):133-8. doi: 10.1097/00005176-198302010-00015.
Suckling rats were injected with thyroid and adrenal cortex hormones to explore their maturative effect on the activities of colon glycosidases. In experiment 1, rats 2, 5, and 8 days old were injected daily with thyroxine (2 micrograms/g body weight) or cortisone acetate (50 micrograms/g body weight) for 3 continuous days. Rats were sacrificed on the 4th day. In experiment 2, rats 4 and 11 days old were injected for 4 continuous days with T3 (0.1 or 0.5 micrograms/g body weight) or a synthetic analog, DIMIT (at the same dose), with sacrifice on the 5th day. Activities of beta-N-acetylglucosaminidase, beta-galactosidase, alpha-fucosidase, and beta-glucuronidase were assayed in homogenates of colon divided into two segments, proximal and distal. Administration of hormones did not influence the protein content of the colonic segments, but exhibited an effect on enzymatic activity, depending on age and on colonic segment. Specific activity of the first three enzymes was precociously decreased in the proximal segment in the younger rats (5 and 8 days old), but not in the older groups (11 and 15 days old). No effect of these hormones was noted on the activities of these enzymes in the distal segment.
给乳鼠注射甲状腺激素和肾上腺皮质激素，以探究它们对结肠糖苷酶活性的成熟作用。在实验1中，对2日龄、5日龄和8日龄的大鼠连续3天每天注射甲状腺素（2微克/克体重）或醋酸可的松（50微克/克体重）。在第4天处死大鼠。在实验2中，对4日龄和11日龄的大鼠连续4天注射T3（0.1或0.5微克/克体重）或一种合成类似物DIMIT（相同剂量），并在第5天处死。在分为近端和远端两个节段的结肠匀浆中测定β-N-乙酰氨基葡萄糖苷酶、β-半乳糖苷酶、α-岩藻糖苷酶和β-葡萄糖醛酸酶的活性。激素给药不影响结肠节段的蛋白质含量，但根据年龄和结肠节段对酶活性有影响。在较年幼的大鼠（5日龄和8日龄）中，近端节段前三种酶的比活性过早降低，但在较年长的组（11日龄和15日龄）中没有。未观察到这些激素对远端节段中这些酶的活性有影响。
