原位杂交显示的特珀摇蚊核仁组织者数量的差异。

Differences in the number of nucleolus organizers in Chironomus tepperi shown by in situ hybridization.

作者信息

Eigenbrod J

出版信息

Chromosoma. 1978 Jun 23;67(1):63-6. doi: 10.1007/BF00285648.

Abstract

Two stocks of Chironomus tepperi could be isolated. One stock, N(IV)+, contains nucleolus organizers in chromosome I and IV, whereas the other one, N(IV)-, shows only one nucleolus in chromosome I. It is demonstrated by in situ hybridization with radioactive rRNA that the absence of the nucleolus in chromosome IV of stock N(IV)- is not related to an inactivation of the nucleolar DNA, as might have been suggested, but is due to the lack of ribosomal cistrons.

摘要

可以分离出两种尖音库蚊。一种品系，N(IV)+，在第一和第四条染色体中含有核仁组织者，而另一种品系，N(IV)-，仅在第一条染色体中显示一个核仁。用放射性rRNA进行原位杂交表明，N(IV)-品系第四条染色体中核仁的缺失与核仁DNA的失活无关（尽管可能有人会这样认为），而是由于缺乏核糖体顺反子。