Onishi S, Miyazawa G, Nishimura Y, Sugiyama S, Yamakawa T, Inagaki H, Katoh T, Itoh S, Isobe K
Pediatrics. 1983 Sep;72(3):399-404.
High-pressure liquid chromatography was used to study the development of blood adrenocortical circadian rhythm in a total of 64 children, ranging in age from 1 month to 15 years. Patients with endocrine diseases, congenital anomalies, and diseases of the central nervous system were excluded from this study. Determination of corticosteroid concentration was possible with 20 to 100 microL of serum. Twenty-four hour patterns were determined at six-hour intervals. A distinct circadian rhythm with an amplitude comparable to that of an adult emerged at approximately 6 months of age.
采用高压液相色谱法对64名年龄在1个月至15岁的儿童进行了血肾上腺皮质昼夜节律发育情况的研究。患有内分泌疾病、先天性畸形和中枢神经系统疾病的患者被排除在本研究之外。使用20至100微升血清即可测定皮质类固醇浓度。每隔6小时测定一次24小时模式。在大约6个月大时出现了明显的昼夜节律，其幅度与成年人相当。
