Chaĭkovskiĭ V S, Zorina A D, Korneva I A, Rogozkin V A
Probl Endokrinol (Mosk). 1983 Jul-Aug;29(4):39-43.
The antiserum against testosterone (T) was obtained and its titer-dependent specificity was studied during the rabbit immunization. The radioimmunoassay for T determination in the human saliva, blood serum and urine was developed. The sensitivity is 6 pg, the variation coefficient within the experiment is 5.3 and between experiments is 10.5%, the standard confirmation is 95 to 100% and dihydrotestosterone cross reaction is 62%. The T content in human biological liquids was studied after various routes of its administration. The highest steroid concentration in the blood is seen 0.5 and 2 hours after the oral and intramuscular administration, exceeding the initial level 2-3 times. The highest T concentrations in the saliva and urine are observed 0.5 and 2, 2 and 24 hours after the oral and intramuscular use, respectively, the levels being 40 to 90 times (oral administration) and 2-3 fold (intramuscular injections) highest versus the initial ones. The changes in the saliva T content indicate the T level in the blood. It was shown that the free and total T levels in sportswomen urine are markedly higher in comparison with those of the females, not going in for sport. The T content in the sportsmen urine is within the limits, characteristic for healthy males.
制备了抗睾酮（T）抗血清，并在兔免疫过程中研究了其效价依赖性特异性。建立了用于测定人唾液、血清和尿液中T的放射免疫分析法。灵敏度为6皮克，实验内变异系数为5.3%，实验间变异系数为10.5%，标准确证率为95%至100%，双氢睾酮交叉反应率为62%。研究了T经不同给药途径后人生物液体中的T含量。口服和肌肉注射后0.5小时和2小时血液中类固醇浓度最高，超过初始水平2至3倍。口服和肌肉注射后，唾液和尿液中T的最高浓度分别在0.5小时和2小时、2小时和24小时观察到，其水平分别比初始水平高40至90倍（口服给药）和2至3倍（肌肉注射）。唾液中T含量的变化表明了血液中的T水平。结果表明，与不从事运动的女性相比，女运动员尿液中游离T和总T水平明显更高。男运动员尿液中的T含量在健康男性的特征范围内。
