Buerkert J, Martin D
Proc Soc Exp Biol Med. 1983 Sep;173(4):533-40. doi: 10.3181/00379727-173-41683.
In this study, the role of nephron recruitment to measurable filtration in the recovery of whole kidney GFR following release of ureteral obstruction of 18 hr duration was characterized in the young rat with a modification of Hanssen's technique. Following release of bilateral (BUO) or unilateral (UUO) ureteral obstruction, the expected increases in fractional sodium and water excretion were observed and remained constant throughout the interval of the study (approximately 3 hr). However, the GFR of the postrelease kidney of rats subjected to BUO and UUO increased significantly within this time frame. Surprisingly, the percent increase in GFR was greater in the the UUO group. In both groups, there was a positive correlation between the number of filtering nephrons and time after release. The increase in the number of filtering juxtamedullary and superficial nephrons detectable with this technique was symmetrical after release of UUO and BUO. However, at all intervals of study, there were significantly fewer filtering nephrons in the postrelease kidney of the UUO and the BUO group. These studies indicate that the mechanism of recovery of GFR in the postrelease kidney in the two settings of ureteral obstruction is quite different.
在本研究中，采用改良的汉森技术，对幼鼠输尿管梗阻18小时后解除梗阻时，肾单位募集对全肾肾小球滤过率（GFR）恢复过程中可测量滤过的作用进行了表征。双侧（BUO）或单侧（UUO）输尿管梗阻解除后，观察到钠和水排泄分数预期增加，且在整个研究期间（约3小时）保持恒定。然而，接受BUO和UUO的大鼠解除梗阻后肾脏的GFR在此时间范围内显著增加。令人惊讶的是，UUO组GFR的增加百分比更大。在两组中，滤过肾单位数量与解除梗阻后的时间呈正相关。用该技术检测到的滤过近髓肾单位和浅表肾单位数量在UUO和BUO解除后呈对称增加。然而，在研究的所有时间段，UUO组和BUO组解除梗阻后肾脏中滤过肾单位明显较少。这些研究表明，在两种输尿管梗阻情况下，解除梗阻后肾脏GFR恢复的机制有很大不同。
