Lyn-Cook B D, Wilson J E
Pharmacol Biochem Behav. 1983 Jun;18(6):949-52. doi: 10.1016/s0091-3057(83)80019-7.
In vitro transfer of 32P from [gamma-32P]-ATP into proteins of particulate fractions from osmotically shocked preparations enriched in rat brain synaptosomes was studied. Phosphate incorporation into protein bands of apparent molecular weights (MW) 44,000, 24,000, 21,000, and 19,000 was affected by the prior experiences of the rats from which the particulate fractions were prepared. Incorporation into all four proteins was increased in particulate fraction from previously naive rats that received active avoidance training. Handling of the subjects prior to training prevented the response of the 24,000 MW protein to training. Phosphate incorporation into 24,000 and 19,000 MW proteins was increased in preparations from previously naive rats that underwent a yoked experience, while incorporation into the 21,000 MW protein was slightly decreased. The yoked experience did not affect in vitro phosphate incorporation into any of these proteins in particulate fractions from previously handled rats.
研究了在体外将[γ-32P]-ATP中的32P转移至富含大鼠脑突触体的经渗透压休克处理的制剂的颗粒部分的蛋白质中。明显分子量（MW）为44,000、24,000、21,000和19,000的蛋白质条带中的磷酸盐掺入受制备颗粒部分所用大鼠先前经历的影响。在接受主动回避训练的先前未经处理的大鼠的颗粒部分中，所有四种蛋白质的掺入均增加。训练前对实验对象的处理阻止了分子量为24,000的蛋白质对训练的反应。在经历了配对体验的先前未经处理的大鼠的制剂中，分子量为24,000和19,000的蛋白质中的磷酸盐掺入增加，而分子量为21,000的蛋白质中的掺入略有减少。配对体验对先前处理过的大鼠的颗粒部分中这些蛋白质的任何一种的体外磷酸盐掺入均无影响。
