出生后早期营养不良会损害大鼠脑突触体中蛋白激酶C依赖性磷酸化作用。

Early postnatal undernutrition impairs protein kinase C-dependent phosphorylation in rat brain synaptosomes.

作者信息

Chakrabarti S, Sonaye B, Nadkarni P P, Da Silva S V

机构信息

Department of Biochemistry, Goa Medical College, Bambolim, India.

出版信息

Neurosci Lett. 1993 Feb 5;150(1):65-7. doi: 10.1016/0304-3940(93)90109-x.

DOI:10.1016/0304-3940(93)90109-x

PMID:8469405

Abstract

Undernutrition in rat pups was established by restricting feeding time daily. Protein kinase C-dependent phosphorylation in vitro was studied by incubating the mitochondrial-synaptosomal membrane fractions from adult, 18-day-old control and undernourished rats with gamma-[32P]ATP in presence of Ca2+ and phosphatidylserine. In adult and 18-day-old control rats, an increased phosphorylation of three major proteins (49, 53, 84 kDa) were detected in presence of calcium and phosphatidylserine. However, in 18-day-old undernourished rats, calcium/phosphatidylserine activated phosphorylation was found to be significantly impaired with only a slightly increased labelling detected in the 49 kDa protein.

摘要

通过每日限制喂食时间来造成幼鼠营养不良。通过在钙离子和磷脂酰丝氨酸存在的情况下，将成年、18日龄对照和营养不良大鼠的线粒体-突触体膜组分与γ-[32P]ATP一起孵育，来研究体外蛋白激酶C依赖性磷酸化。在成年和18日龄对照大鼠中，在钙离子和磷脂酰丝氨酸存在的情况下，检测到三种主要蛋白质（49、53、84 kDa）的磷酸化增加。然而，在18日龄营养不良大鼠中，发现钙/磷脂酰丝氨酸激活的磷酸化显著受损，仅在49 kDa蛋白质中检测到轻微增加的标记。