Shihabi Z K, Andrews R I, Scaro J
Clin Chim Acta. 1982 Sep 1;124(1):75-83. doi: 10.1016/0009-8981(82)90321-7.
Cortisol in concentrated sulfuric acid forms two major fluorescing compounds which are separated by chromatography on a reversed-phase column. Based on this reaction, urine free cortisol, after double extraction was assayed by high-performance liquid chromatography with fluorescent detection. The method is sensitive, rapid, and free from interferences. The values by this method are 30% lower than radioimmunoassay. In addition to Cushing's syndrome, patients with acute pancreatitis had elevated levels of urinary free cortisol which parallel urinary amylase levels while patients with myocardial infarction had normal levels. Urinary free cortisol showed diurnal variation with elevated values early in the morning.
皮质醇在浓硫酸中形成两种主要的荧光化合物，通过反相柱色谱法进行分离。基于此反应，对经过两次萃取的尿游离皮质醇采用带荧光检测的高效液相色谱法进行测定。该方法灵敏、快速且无干扰。用此方法测得的值比放射免疫测定法低30%。除库欣综合征外，急性胰腺炎患者的尿游离皮质醇水平升高，与尿淀粉酶水平平行，而心肌梗死患者的尿游离皮质醇水平正常。尿游离皮质醇呈现昼夜变化，清晨时水平升高。
