Schöneshöfer M, Fenner A, Dulce H J
Clin Chim Acta. 1980 Feb 14;101(1):125-34. doi: 10.1016/0009-8981(80)90063-7.
The magnitude of interferences arising in the radioimmunological assessment of urinary free cortisol is studied (a) by comparing cortisol immunoreactivities from crude urine, after organic solvent extraction of different selectivity and after additional chromatography by high pressure liquid chromatography (HPLC) and (b) by evaluation of the profile of immunoreactivity resulting from the fractions eluted by HPLC. Three antisera from different sources have been investigated. Values of cortisol-immunoreactivity in crude urine were about six times and values of a simple dichloromethane extract about three times higher than values obtained after HPLC. The main part of the interfering compounds arising in organic extracts have a polarity similar to cortisol, which cannot be easily eliminated by simple solvent extraction procedures. Specific estimation of urinary cortisol by radioimmunoassay requires a preceding chromatographic technique of high efficiency, such as HPLC, which represents an adequate tool for the routine laboratory.
(a) 通过比较不同选择性有机溶剂萃取后的粗尿、经高压液相色谱 (HPLC) 进一步色谱分析后的皮质醇免疫反应性；(b) 通过评估HPLC洗脱组分产生的免疫反应性谱图。研究了三种不同来源的抗血清。粗尿中的皮质醇免疫反应性值约为HPLC后所得值的六倍，简单二氯甲烷提取物的值约为其三倍。有机提取物中产生的干扰化合物的主要部分具有与皮质醇相似的极性，简单的溶剂萃取程序不易将其去除。通过放射免疫法对尿皮质醇进行特异性测定需要高效的前期色谱技术，如HPLC，它是常规实验室的合适工具。
