Sekiya S, Iwasawa H, Takamizawa H, Sugita M, Tenjin Y
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Sep;34(9):1487-90.
The survival of asynchronous rat ovarian adenocarcinoma cells (ROT68/C1) treated for 1 hr with more than 0.5 microgram/ml of cis-diamminedichloroplatinum (II) (Cisplatin) revealed a marked decrease in a dose-dependent manner, as estimated by the vitro colonyforming method. Although the ROT68/C1 cells showed no significant sensitivity to 0.1 microgram/ml of Cisplatin for 1 hr, more than 50% of the cells were killed when the exposure interval was prolonged for 16 hr. The decrease in percent survival of cells with increasing Cisplatin concentration was parallel to that of the uptake of 3H-thymidine, whereas the uptake of 3H-uridine was not inhibited in a dose-dependent manner. The DNA histogram pattern of nontreated asynchronous ROT68/C1 cells was distributed widely and a peak of accumulation was observed near 6C. When the cells were exposed to more than 0.5 microgram/ml of Cisplatin for 1 hr, the accumulation of cells near 2C was observed to occur more rapidly with increasing concentration. The cells distributed near 2C appeared to be cells with karyorrhexis and damaged irreversibly.
用高于0.5微克/毫升的顺二氨二氯铂（II）（顺铂）处理异步大鼠卵巢腺癌细胞（ROT68/C1）1小时，通过体外集落形成法评估，其存活率呈剂量依赖性显著下降。尽管ROT68/C1细胞对0.1微克/毫升的顺铂处理1小时无明显敏感性，但当暴露时间延长至16小时时，超过50%的细胞被杀死。随着顺铂浓度增加，细胞存活率的降低与3H-胸腺嘧啶核苷摄取量的降低平行，而3H-尿苷的摄取未呈剂量依赖性抑制。未处理的异步ROT68/C1细胞的DNA直方图模式分布广泛，在6C附近观察到一个积累峰值。当细胞用高于0.5微克/毫升的顺铂处理1小时时，随着浓度增加，在2C附近的细胞积累出现得更快。分布在2C附近的细胞似乎是核碎裂且不可逆损伤的细胞。
