来自短乳杆菌野生型的抗奇霉素延伸因子Tu

Kirromycin-resistant elongation factor Tu from wild-type of Lactobacillus brevis.

作者信息

Wörner W, Wolf H

出版信息

FEBS Lett. 1982 Sep 20;146(2):322-6. doi: 10.1016/0014-5793(82)80944-7.

Abstract

Properties of the elongation factor Tu from Lactobacillus brevis which is naturally insensitive to kirromycin are described. The protein is characterized by an unusual nucleotide-binding site with increased affinity for GTP and extreme heat stability. EF-Tu is sensitive to pulvomycin in the assay of polyphenylalanine synthesis. However, the failure of the protein to display pulvomycin-dependent GDP-binding and GTPase activity indicates that pulvomycin action in L. brevis differs from that in E. coli.

摘要

描述了来自短乳杆菌的延伸因子Tu的特性，该因子对奇霉素天然不敏感。该蛋白质的特征在于具有对GTP亲和力增加和极高热稳定性的异常核苷酸结合位点。在聚苯丙氨酸合成测定中，EF-Tu对普尔霉素敏感。然而，该蛋白质未能表现出普尔霉素依赖性GDP结合和GTP酶活性，这表明普尔霉素在短乳杆菌中的作用与在大肠杆菌中的作用不同。