Zhirnov O P, Bukrinskaia A G
Vopr Virusol. 1982 Sep-Oct;27(5):37-44.
Incorporation of individual viral proteins (P1, P2, P3, NP, NA, HA, and M) into virions was studied by the "pulse-chase" method followed by electrophoretic analysis of the proteins in chick fibroblast cultures infected with influenza WSN virus. Viral proteins were found to incorporate into virions rather rapidly and for a long time after synthesis, with the exception of M protein incorporation of which into the virus markedly decreased by 4 hours after synthesis. Nucleocapsid proteins (NP, P1, P2, and P3) incorporated into the virus asynchronously: by 17 hours postinoculation, in NP protein prevailed the molecules synthesized after 6 hours of infection, in P2 protein the molecules synthesized in the first hours of infection, and P1 and P3 proteins were represented by a uniform set of molecules synthesized at various periods after inoculation. The evidence on heterogeneity of P2 and M proteins in the virus has been obtained. Possible mechanisms regulating the selection of molecules of individual viral proteins in formation of virus particles are discussed.
通过“脉冲追踪”方法，随后对感染流感WSN病毒的鸡成纤维细胞培养物中的蛋白质进行电泳分析，研究了单个病毒蛋白（P1、P2、P3、NP、NA、HA和M）掺入病毒粒子的情况。发现病毒蛋白在合成后相当迅速且长时间地掺入病毒粒子，但M蛋白除外，其掺入病毒的情况在合成后4小时明显减少。核衣壳蛋白（NP、P1、P2和P3）异步掺入病毒：接种后17小时，NP蛋白中感染6小时后合成的分子占优势，P2蛋白中感染最初几小时合成的分子占优势，P1和P3蛋白由接种后不同时期合成的一组均匀分子代表。已获得病毒中P2和M蛋白异质性的证据。讨论了在病毒颗粒形成过程中调节单个病毒蛋白分子选择的可能机制。
