神经丝与微管相关蛋白之间的相互作用：一种细胞器内桥接的可能机制。
Interactions between neurofilaments and microtubule-associated proteins: a possible mechanism for intraorganellar bridging.
作者信息
Leterrier J F, Liem R K, Shelanski M L
出版信息
J Cell Biol. 1982 Dec;95(3):982-6. doi: 10.1083/jcb.95.3.982.
Abstract
Mammalian neurofilaments prepared from brain and spinal cord by either of two methods partially inhibit the in vitro assembly of microtubules. This inhibition is shown to be due to the association of a complex of high molecular weight microtubule-associated proteins (MAP1 and MAP2) and tubulin with the neurofilament. Further analysis of the association reveals a saturable binding of purified brain MAPs to purified neurofilaments with a Kd of 10(-7) M. Purified astroglial filaments neither inhibit microtubule assembly nor show significant binding of MAPs. It is proposed that the MAPs might function as one element in a network of intraorganellar links in the cytoplasm.
摘要
通过两种方法之一从大脑和脊髓制备的哺乳动物神经丝会部分抑制微管的体外组装。这种抑制作用被证明是由于高分子量微管相关蛋白（MAP1和MAP2）与微管蛋白的复合物与神经丝结合所致。对这种结合的进一步分析表明，纯化的脑MAP与纯化的神经丝存在可饱和结合，解离常数为10^(-7) M。纯化的星形胶质细胞丝既不抑制微管组装，也未显示出MAP的显著结合。有人提出，MAP可能作为细胞质中细胞器内连接网络的一个元素发挥作用。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Cytoplasmic network arrays demonstrated by immunolocalization using antibodies to a high molecular weight protein present in cytoskeletal preparations from cultured cells.利用针对存在于培养细胞细胞骨架制剂中的一种高分子量蛋白质的抗体，通过免疫定位法显示的细胞质网络阵列。
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ATP-induced formation of an associated complex between microtubules and neurofilaments.三磷酸腺苷（ATP）诱导微管与神经丝之间形成相关复合物。
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