Liem R K, Hutchison S B
Biochemistry. 1982 Jun 22;21(13):3221-6. doi: 10.1021/bi00256a029.
Mammalian neurofilaments are composed of three subunit polypeptides with approximate molecular weights of 200 000, 150 000, and 70 000 (P200, P150, and P70). These subunits were separated by ion-exchange chromatography in the presence of 8 M urea. The P200 polypeptide was differentially eluted on a diethylaminoethyl (DEAE) column. The P70 and P150 polypeptides obtained after the DEAE column were separable on a hydroxylapatite column. Under neurofilament assembly conditions, only the P70 polypeptide was able to reassemble into an intermediate filament in the absence of the other two polypeptides. The P150 and P70 polypeptides copolymerized into an intermediate filament, only if P70 was present. These results suggest that the P70 polypeptide forms the core of the filament and the other two polypeptides are tightly associated accessory proteins.
哺乳动物神经丝由三种亚基多肽组成，其近似分子量分别为200000、150000和70000（P200、P150和P70）。这些亚基在8M尿素存在下通过离子交换色谱法分离。P200多肽在二乙氨基乙基（DEAE）柱上被差异洗脱。DEAE柱后获得的P70和P150多肽在羟基磷灰石柱上可分离。在神经丝组装条件下，只有P70多肽能够在没有其他两种多肽的情况下重新组装成中间丝。只有当P70存在时，P150和P70多肽才会共聚成中间丝。这些结果表明，P70多肽形成了丝的核心，而其他两种多肽是紧密相关的辅助蛋白。
