Littlefield J W, Felix J S
Somatic Cell Genet. 1982 Nov;8(6):743-57. doi: 10.1007/BF01543016.
A new clonal CBA mouse teratocarcinoma cell line called H6 grows rapidly without calcium in suspension culture. When attached to a gelatin-coated surface in the presence of calcium, differentiation to large flat cells, which secrete plasminogen activator, occurs after exposure to retinoic acid. Differentiation is terminal after about six cell divisions. H6 variants resistant to 6-thioguanine, 5-bromodeoxyuridine, ouabain, chloramphenicol, retinoic acid, or combinations thereof, have been isolated. Cell hybridizations were made between undifferentiated stem cells and the differentiated derivatives of H6. These experiments indicate that the undifferentiated cells can "rescue" cells in the initial stages of differentiation and that the signs of differentiation are soon lost in such hybrids. Hybridization without "rescue" may also occur, as suggested by small abortive colonies of large cells.
一种名为H6的新的克隆CBA小鼠畸胎瘤细胞系，在无钙的悬浮培养中生长迅速。当在有钙的情况下附着于明胶包被的表面时，在暴露于视黄酸后会分化为分泌纤溶酶原激活剂的大的扁平细胞。在大约六次细胞分裂后分化终止。已经分离出对6-硫鸟嘌呤、5-溴脱氧尿苷、哇巴因、氯霉素、视黄酸或其组合具有抗性的H6变体。在未分化的干细胞与H6的分化衍生物之间进行了细胞杂交。这些实验表明，未分化的细胞可以在分化的初始阶段“拯救”细胞，并且在这种杂交体中分化的迹象很快就会消失。如大细胞的小流产集落所示，也可能发生没有“拯救”的杂交。
