Verheijen J H, Mullaart E, Chang G T, Kluft C, Wijngaards G
Thromb Haemost. 1982 Dec 27;48(3):266-9.
An indirect spectrophotometric assay for extrinsic plasminogen activator has been devised, which is based on the parabolic assay of Drapier et al. (5). The system contains activator, plasminogen, the synthetic plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251, Kabi) and a mixture of soluble fibrinogen fragments prepared by treatment of fibrinogen with cyanogen bromide. The addition of these fibrinogen fragments considerably enhances the sensitivity and specificity of the method owing to specific stimulation of the plasminogen activation by extrinsic plasminogen activator. The assay conditions were optimized and the application for extrinsic plasminogen activator measurements in plasma euglobulin fractions is demonstrated.
已设计出一种用于外源性纤溶酶原激活剂的间接分光光度测定法，该方法基于Drapier等人（5）的抛物线测定法。该系统包含激活剂、纤溶酶原、合成纤溶酶底物H-D-缬氨酸-亮氨酸-赖氨酸-对硝基苯胺（S-2251，卡比）以及通过用溴化氰处理纤维蛋白原制备的可溶性纤维蛋白原片段混合物。由于外源性纤溶酶原激活剂对纤溶酶原激活的特异性刺激，这些纤维蛋白原片段的加入大大提高了该方法的灵敏度和特异性。对测定条件进行了优化，并展示了该方法在血浆优球蛋白组分中外源性纤溶酶原激活剂测量中的应用。
