肝片吸虫卵的分离与纯化

Isolation and purification of Fasciola hepatica eggs.

作者信息

Reddington J J, Leid R W, Wescott R B

出版信息

Vet Parasitol. 1982 Nov;11(2-3):179-83. doi: 10.1016/0304-4017(82)90040-1.

Abstract

The present egg isolation method is both a rapid and simple technique for recovering large numbers of Fasciola hepatica eggs (1 X 10(7) eggs/gradient) from bovine bile. Bile from infected cattle was first passed through a 45 micron screen sieve. The F. hepatica eggs were collected from the surface of the screen by backwashing with a jet of distilled water. The resultant egg suspension was layered on a 60% to 100% (v/v) linear Percoll gradient prepared in distilled water. Centrifugation at 450 g for 20 min resulted in the formation of 2 visible bands and a pellet. The top band (density of 1.075 g ml-1) contained viscous debris and crystallized bile pigments. The second visible band (density of 1.093-1.099 g ml-1) consisted of a relatively pure population of F. hepatica eggs (greater than 93%) while the pellet contained only F. hepatica egg shells.

摘要

目前的虫卵分离方法是一种从牛胆汁中快速、简便地获取大量肝片吸虫虫卵（1×10⁷个虫卵/梯度）的技术。首先将感染牛的胆汁通过一个45微米的筛网。用一股蒸馏水反冲洗筛网表面来收集肝片吸虫虫卵。将所得的虫卵悬液铺在由蒸馏水配制的60%至100%（v/v）线性 Percoll 梯度上。以450 g 离心20分钟会形成两条可见带和一个沉淀。顶部条带（密度为1.075 g/ml）包含粘性碎片和结晶胆汁色素。第二条可见带（密度为1.093 - 1.099 g/ml）由相对纯净的肝片吸虫虫卵群体（超过93%）组成，而沉淀仅包含肝片吸虫的卵壳。