Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil.
Parasitol Res. 2012 Feb;110(2):663-7. doi: 10.1007/s00436-011-2538-6. Epub 2011 Jul 20.
Fasciolosis is a disease caused by Fasciola hepatica responsible for causing significant losses in livestock. This study aimed to evaluate the Pochonia chlamydosporia fungus (isolate VC1) on F. hepatica eggs after passing through the cattle gastrointestinal tract. For this evaluation, 1 g pellet was given in sodium alginate matrix per kilogram live weight containing 25% of fungal mycelium from isolate VC1 per animal. Twelve animals were used, six treated and six untreated (control). Some stool samples were collected from the groups of treated and control animals, at the times of 12, 18, 24, 48, 72, and 96 h after the pellets' administration. Then, from each stool sample of treated and control groups, 2 g was placed in a Petri dish of 9 cm in diameter, containing 2% water-agar and 1,000 eggs of F. hepatica. It was observed that the fungus was effective in preying upon the eggs in the samples recovered at all of the schedules starting at 12 h. Furthermore, differences were observed (p < 0.01) in the destruction of eggs in the Petri dishes in the treated group compared with the control group. The ovicidal effect was observed after 7 days of interaction. The ovicidal P. chlamydosporia fungus was effective in destroying F. hepatica eggs; therefore, it is suggested that this fungus could be employed as agent for the control of helminth eggs.
片形吸虫病是一种由肝片吸虫引起的疾病，可导致家畜大量损失。本研究旨在评估经过牛胃肠道后对肝片形吸虫卵的多形拟青霉（分离株 VC1）。为此，每公斤活重用海藻酸钠基质给予 1 g 丸剂，每头动物含 25%的真菌菌丝体来自分离株 VC1。使用了 12 只动物，6 只接受治疗，6 只未接受治疗（对照）。在给予丸剂后 12、18、24、48、72 和 96 小时，从治疗组和对照组的一些粪便样本中采集了一些粪便样本。然后，从每组粪便样本中取出 2 g，放入直径为 9 cm 的培养皿中，含有 2%水琼脂和 1000 个肝片形吸虫卵。结果表明，从 12 小时开始，在所有时间点回收的样本中，真菌对卵的捕食都是有效的。此外，与对照组相比，在处理组的培养皿中观察到卵的破坏存在差异（p<0.01）。在 7 天的相互作用后观察到杀卵作用。杀卵多形拟青霉对肝片形吸虫卵有效，因此建议该真菌可用作控制寄生虫卵的制剂。
