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用对流免疫电泳法鉴别几种巨大消化球菌分离株。

Differentiation of several isolates of Peptococcus magnus by counterimmunoelectrophoresis.

作者信息

Markowitz A, Lerner A M

出版信息

Infect Immun. 1977 Apr;16(1):152-4. doi: 10.1128/iai.16.1.152-154.1977.

Abstract

Uniform sonicated suspensions of three clinical isolates of Peptococcus magnus that were morphologically, chromatographically, and biochemically identical were used to produce hyperimmune antisera in rabbits. Similar hyperimmune serum was prepared to a single strain of Peptostreptococcus anaerobius. When these antisera and antigens were tested by counterimmunoelectrophoresis, the three isolates of Peptococcus magnus were immunologically distinct. None of the antisera to Peptococcus magnus formed precipitin lines with Peptostreptococcus anaerobius.

摘要

使用形态学、色谱法和生物化学性质均相同的三株巨大消化球菌临床分离株的均匀超声处理悬浮液,在兔体内制备超免疫抗血清。针对一株厌氧消化链球菌也制备了类似的超免疫血清。当通过对流免疫电泳对这些抗血清和抗原进行检测时,三株巨大消化球菌分离株在免疫上是不同的。针对巨大消化球菌的抗血清均未与厌氧消化链球菌形成沉淀线。

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