Wong M, Catena A, Hadley W K
J Clin Microbiol. 1980 May;11(5):515-21. doi: 10.1128/jcm.11.5.515-521.1980.
Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-reactions in heterologous antigen-antibody combinations were eliminated, leaving only species-specific precipitin arcs. These absorbed antisera, specific for each Peptostreptococcus species by Ouchterlony tests, were used for rapid identification studies. Staphylococcus aureus-bearing protein A was sensitized with each absorbed antiserum. These reagents produced specific coagglutination reactions with suspensions of each Peptostreptococcus reference strain and with 16 clinical isolates. No cross-reactions occurred with the Streptococcus intermedius, Peptococcus magnus, or Peptococcus asaccharolyticus strains tested.
针对每种消化链球菌属细菌（厌氧消化链球菌、微小消化链球菌、短小消化链球菌和产酶消化链球菌）的全细胞抗血清在兔体内制备。当这些抗血清在双向琼脂扩散试验中与经超声破碎的细胞和培养上清液反应时，所有菌种的抗原与未接种的培养基之间出现了同一性关系线。随后，当抗血清用用于培养消化链球菌的脱水培养基吸收时，异源抗原 - 抗体组合中的所有交叉反应均被消除，仅留下种特异性沉淀弧。这些经双向琼脂扩散试验对每种消化链球菌属细菌具有特异性的吸收抗血清用于快速鉴定研究。携带蛋白A的金黄色葡萄球菌用每种吸收抗血清致敏。这些试剂与每种消化链球菌参考菌株的悬液以及16株临床分离株产生了特异性协同凝集反应。与所测试的中间链球菌、大消化球菌或解糖消化球菌菌株未发生交叉反应。
