Bergqvist Y, Larsson K, Hansen E L
Clin Chim Acta. 1980 Mar 14;102(1):67-74. doi: 10.1016/0009-8981(80)90434-9.
A new method for determination of palmitic acid in amniotic fluid was developed. The dipalmitoyl lecithin was hydrolysed, and after extraction the palmitic acid was flash methylated in the injector of the gas chromatograph. The between batch coefficient of variation was 6.0% at 40 and 70 mumol/l of palmitic acid concentrations in 500 microliters amniotic fluid. The accuracy of the method was demonstrated by linearity, recovery and correlation studies. The correlation coefficient was 0.98 between the new and bortrifluoride methylating method. The concentrations of lecithin and palmitic acid in amniotic fluid showed a correlation coefficient of 0.97. If the concentration of palmitic acid falls below 80 mumol/l respiration distress syndrome is likely. The new method was simple and easy to carry out and could be adjusted for haemoglobin contamination of the amniotic fluid. It was concluded that the determination of palmitic acid in amniotic fluid was a reliable method for the assessment of fetal lung maturity that could replace the determination of lecithin concentration.
开发了一种测定羊水棕榈酸的新方法。二棕榈酰卵磷脂被水解，提取后，棕榈酸在气相色谱仪的进样器中进行快速甲基化。在500微升羊水中，棕榈酸浓度为40和70微摩尔/升时，批次间变异系数为6.0%。通过线性、回收率和相关性研究证明了该方法的准确性。新方法与三氟硼酸盐甲基化方法之间的相关系数为0.98。羊水中卵磷脂和棕榈酸的浓度相关系数为0.97。如果棕榈酸浓度低于80微摩尔/升，则可能发生呼吸窘迫综合征。该新方法简单易行，可针对羊水血红蛋白污染进行调整。得出的结论是，测定羊水中的棕榈酸是评估胎儿肺成熟度的可靠方法，可替代卵磷脂浓度的测定。
