Golden P, Greenwalt A, Martin K, Bellorin-Font E, Mazey R, Klahr S, Slatopolsky E
Endocrinology. 1980 Aug;107(2):602-7. doi: 10.1210/endo-107-2-602.
Because of differing reports of an effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on parathyroid hormone (PTH) secretion, the present studies were performed in vitro using bovine parathyroid gland slices and isolated parathyroid cells. Both COOH-terminal and NH2-terminal RIAs for PTH were employed. No effect of 1,25(OH)2D3 on PTH secretion was found during a 4-h incubation of parathyroid slices in variable external calcium concentrations. The results from dual RIA measurements also showed no effect of 1,25(OH)2D3 on PTH secretion by isolated parathyroid cells during 90- to 150-min incubations with variable calcium concentrations. In addition, extensive analysis by polyacrylamide gel electrophoresis showed no effect of 1,25(OH)2D3 on cAMP generation. Whereas calcium inhibited and isoproterenol stimulated the production of cAMP, 1,25(OH)2D3 had no effect. We conclude that 1,25(OH)2D3 does not affect parathyroid gland function acutely in vitro.
由于关于1,25 - 二羟基维生素D3[1,25(OH)2D3]对甲状旁腺激素（PTH）分泌影响的报道存在差异，本研究采用牛甲状旁腺切片和分离的甲状旁腺细胞进行体外实验。同时使用了PTH的羧基末端和氨基末端放射免疫分析法（RIAs）。在不同外部钙浓度下对甲状旁腺切片进行4小时孵育期间，未发现1,25(OH)2D3对PTH分泌有影响。双重放射免疫分析测量结果也显示，在不同钙浓度下对分离的甲状旁腺细胞进行90至150分钟孵育期间，1,25(OH)2D3对PTH分泌没有影响。此外，通过聚丙烯酰胺凝胶电泳进行的广泛分析表明，1,25(OH)2D3对环磷酸腺苷（cAMP）的生成没有影响。虽然钙抑制而异丙肾上腺素刺激cAMP的产生，但1,25(OH)2D3没有作用。我们得出结论，在体外1,25(OH)2D3不会急性影响甲状旁腺功能。
