Rudberg C, Akerström G, Johansson H, Ljunghall S, Malmaeus J, Wide L
Acta Endocrinol (Copenh). 1984 Mar;105(3):354-9. doi: 10.1530/acta.0.1050354.
The effects of 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) and 24,25-dihydroxycholecalciferol (24,25-(OH)2D3) on parathyroid hormone (PTH) release from human parathyroid cells were investigated using an in vitro system of dispersed cells. The cells were obtained from 7 patients with primary hyperparathyroidism (HPT) and adenoma, 4 patients with primary HPT due to hyperplasia and 2 patients with parathyroid hyperplasia secondary to chronic renal failure. The dispersed cells were incubated in tissue culture medium at low, normal and high external calcium concentrations for 2-16 h. There was a gradual suppression of PTH release (5-55%) when the calcium concentration in the medium was increased from 0.5 to 3.0 mM, thus indicating retained regulation of hormone release. The addition of 1,25-(OH)2D3 in concentrations of 0.1 and 1 ng/ml and of 24,25-(OH)2D3 in concentrations of 1.0 and 10 ng/ml during the incubations did not further affect the amount of PTH released by the cells. The concentrations of the different vitamin D metabolites tested closely correspond to levels observed under normal physiological conditions and during treatment with high doses of vitamin D in vivo. Thus, the findings contradict the idea of any direct short-term regulatory effect of either 1,25-(OH)2D3 or 24,25-(OH)2D3 on the secretion of PTH from hyperfunctioning human parathyroid tissue.
利用分散细胞的体外系统，研究了1,25 - 二羟胆钙化醇（1,25-(OH)₂D₃）和24,25 - 二羟胆钙化醇（24,25-(OH)₂D₃）对人甲状旁腺细胞甲状旁腺激素（PTH）释放的影响。细胞取自7例原发性甲状旁腺功能亢进症（HPT）伴腺瘤患者、4例增生性原发性HPT患者以及2例慢性肾衰竭继发性甲状旁腺增生患者。将分散细胞在低、正常和高细胞外钙浓度的组织培养基中孵育2 - 16小时。当培养基中的钙浓度从0.5 mM增加到3.0 mM时，PTH释放逐渐受到抑制（5 - 55%），这表明激素释放的调节功能得以保留。在孵育过程中添加浓度为0.1和1 ng/ml的1,25-(OH)₂D₃以及浓度为1.0和10 ng/ml的24,25-(OH)₂D₃，并未进一步影响细胞释放的PTH量。所测试的不同维生素D代谢物的浓度与正常生理条件下以及体内高剂量维生素D治疗期间观察到的水平密切对应。因此，这些发现与1,25-(OH)₂D₃或24,25-(OH)₂D₃对功能亢进的人甲状旁腺组织分泌PTH有任何直接短期调节作用的观点相矛盾。
