Ohuchi K, Sato H, Komabayashi T, Tsurufuji S, Satoh H, Levine L
Prostaglandins Med. 1980 Oct;5(4):267-74. doi: 10.1016/0161-4630(80)90029-4.
Seven-day-old carrageenin granuloma tissue of rats, when incubated in organ culture, produced 6-keto-prostaglandin F1 alpha, prostaglandin E2, prostaglandin F2 alpha, and thromboxane B2 in ratios of 16/7/1/1, respectively. Indomethacin (1 microgram/ml) inhibited this cyclooxygenase-mediated arachidonic acid metabolism. Dexamethasone (1 microgram/ml) also inhibited this metabolic cascade. Its inhibitory effect was not immediate; preincubation of the tissue with dexamethasone potentiated its inhibitory effectiveness. Cycloheximide (0.1 to 10 microgram/ml) also inhibited prostaglandin production. The inhibitory mechanism by cycloheximide of prostaglandin production was discussed in relation to that by dexamethasone.
将大鼠的角叉菜胶肉芽肿组织在器官培养中孵育7天后，分别以16/7/1/1的比例产生6-酮-前列腺素F1α、前列腺素E2、前列腺素F2α和血栓素B2。吲哚美辛（1微克/毫升）抑制这种环氧化酶介导的花生四烯酸代谢。地塞米松（1微克/毫升）也抑制这种代谢级联反应。其抑制作用并非即时产生；用地塞米松对组织进行预孵育可增强其抑制效果。环己酰亚胺（0.1至10微克/毫升）也抑制前列腺素的产生。就地塞米松对前列腺素产生的抑制机制而言，讨论了环己酰亚胺对前列腺素产生的抑制机制。
