大鼠角叉菜胶肉芽肿组织碎块产生前列腺素及其受地塞米松和环己酰亚胺抑制的情况。

Prostaglandin production by minced carrageenin granuloma tissue of rats and its inhibition by dexamethasone and cycloheximide.

作者信息

Ohuchi K, Sato H, Komabayashi T, Tsurufuji S, Satoh H, Levine L

出版信息

Prostaglandins Med. 1980 Oct;5(4):267-74. doi: 10.1016/0161-4630(80)90029-4.

Abstract

Seven-day-old carrageenin granuloma tissue of rats, when incubated in organ culture, produced 6-keto-prostaglandin F1 alpha, prostaglandin E2, prostaglandin F2 alpha, and thromboxane B2 in ratios of 16/7/1/1, respectively. Indomethacin (1 microgram/ml) inhibited this cyclooxygenase-mediated arachidonic acid metabolism. Dexamethasone (1 microgram/ml) also inhibited this metabolic cascade. Its inhibitory effect was not immediate; preincubation of the tissue with dexamethasone potentiated its inhibitory effectiveness. Cycloheximide (0.1 to 10 microgram/ml) also inhibited prostaglandin production. The inhibitory mechanism by cycloheximide of prostaglandin production was discussed in relation to that by dexamethasone.

摘要

将大鼠的角叉菜胶肉芽肿组织在器官培养中孵育7天后，分别以16/7/1/1的比例产生6-酮-前列腺素F1α、前列腺素E2、前列腺素F2α和血栓素B2。吲哚美辛（1微克/毫升）抑制这种环氧化酶介导的花生四烯酸代谢。地塞米松（1微克/毫升）也抑制这种代谢级联反应。其抑制作用并非即时产生；用地塞米松对组织进行预孵育可增强其抑制效果。环己酰亚胺（0.1至10微克/毫升）也抑制前列腺素的产生。就地塞米松对前列腺素产生的抑制机制而言，讨论了环己酰亚胺对前列腺素产生的抑制机制。