Marsili G, Tacconi R, Cipriani S
Ric Clin Lab. 1980 Apr-Jun;10(2):423-9. doi: 10.1007/BF02905352.
A colorimetric procedure is described for the determination of serum iron and TIBC, utilizing the action of the iron on the oxidation reaction of 4-aminophenazone with H2O2. The chromogen oxidized has maximum absorption at 540 nm. The method is interesting because it has important advantages over other methods: no deproteinization is needed, microsamples are used, it is simple, gives adequate precision, and the results correlate well with those obtained with the standard bathophenanthroline method.
本文描述了一种比色法，用于测定血清铁和总铁结合力（TIBC），该方法利用铁对4-氨基安替比林与过氧化氢氧化反应的作用。氧化后的显色剂在540nm处有最大吸收。该方法很有意义，因为与其他方法相比具有重要优势：无需去蛋白，使用微量样本，操作简单，精密度良好，且结果与标准邻二氮菲法所得结果相关性良好。
