Ryan S F, Hashim S A, Cernansky G, Barrett C R, Bell A L, Liau D F
J Lipid Res. 1980 Nov;21(8):1004-14.
We quantified total phospholipid (PL), total and disaturated phosphatidylcholine (PC and DSPC), phosphatidylglycerol (PG), and total protein in alveolar washings and lung tissue in 22 dog lungs. Quantitative recovery of alveolar material and assessment of its possible contamination by blood lipids were important determinants of methodology. To remove blood, the vessels of half the lungs were perfused with a fluorocarbon emulsion before lavage. The volume of blood removed by perfusion and the quantity and fatty acid patterns of its whole blood and plasma PL and PC were determined. Washings of unperfused lungs contained means of 21% more PL and 24% more PC than those of perfused lungs. Although this excess could be accounted for by the PL and PC in pulmonary blood, the hemoglobin and total protein content of washings and their PC fatty acid patterns indicated that blood lipids were not a major source of the excess lipid in washings of unperfused lungs. Using more recent morphometric estimates rather than the indirect ones previously used by others, the quantity of alveolar DSPC (1 mg/g lung) is calculated to be 1.8 times the amount necessary to form a packed monolayer on the internal surface of the lung at functional residual capacity.
我们对22只犬肺的肺泡灌洗液和肺组织中的总磷脂（PL）、总饱和及二饱和磷脂酰胆碱（PC和DSPC）、磷脂酰甘油（PG）和总蛋白进行了定量分析。肺泡物质的定量回收及其可能被血脂污染的评估是方法学的重要决定因素。为了去除血液，在灌洗前用氟碳乳剂灌注一半肺的血管。测定了灌注去除的血液量及其全血和血浆PL和PC的数量及脂肪酸模式。未灌注肺的灌洗液中PL含量比灌注肺的灌洗液平均多21%，PC含量多24%。虽然这种过量可以由肺血中的PL和PC来解释，但灌洗液中的血红蛋白和总蛋白含量及其PC脂肪酸模式表明，血脂不是未灌注肺灌洗液中过量脂质的主要来源。使用比其他人以前使用的间接方法更新的形态测量估计方法，计算出肺泡DSPC的量（1毫克/克肺）是在功能残气量时在肺内表面形成紧密单分子层所需量的1.8倍。
