有机硅烷涂层和表面活性剂可降低温石棉对人肺泡巨噬细胞的体外细胞毒性。

In vitro cytotoxicity of chrysotile asbestos to human pulmonary alveolar macrophages is decreased by organosilane coating and surfactant.

作者信息

Morrison D G, McLemore T L, Lawrence E C, Feuerbacher D G, Mace M L, Busbee D L, Griffin A C, Marshall M V

机构信息

Baylor College of Medicine, Houston, Texas.

出版信息

Cell Biol Toxicol. 1986 Jun;2(2):293-309. doi: 10.1007/BF00122697.

DOI:10.1007/BF00122697

PMID:2855798

Abstract

Human pulmonary alveolar macrophages were used to quantitate the cytotoxic effect of surface-altered chrysotile asbestos. Little difference was observed in mortality between chrysotile asbestos that was surface-treated to a 42% extent by a hydrophobic organosilane or untreated chrysotile. Little or no effect on mortality was observed when human pulmonary alveolar macrophages were cultured with untreated chrysotile or acid-leached asbestos in the presence of 10 mM dipalmitoyl lecithin. However, when human pulmonary alveolar macrophages were cultured with a hydrophobically-treated (to a 42% or 95% extent) chrysotile asbestos in the presence of 10 mM dipalmitoyl lecithin, a statistically significant decrease in mortality was observed compared to untreated chrysotile. No mutagenic activity was observed when V79 cells were cultured with acid-leached, or 42% hydrophobically-treated chrysotile asbestos, even when human pulmonary alveolar macrophages were included as an activation source. The 95% hydrophobically-treated and acid-leached chrysotile also exhibited decreased binding of benzo[a]pyrene compared to untreated chrysotile asbestos.

摘要

人类肺泡巨噬细胞被用于定量表面改性温石棉的细胞毒性作用。经疏水性有机硅烷进行42%程度表面处理的温石棉与未处理的温石棉相比，在死亡率方面观察到的差异很小。当人类肺泡巨噬细胞在10 mM二棕榈酰卵磷脂存在的情况下与未处理的温石棉或酸浸石棉一起培养时，对死亡率几乎没有影响。然而，当人类肺泡巨噬细胞在10 mM二棕榈酰卵磷脂存在的情况下与经疏水处理（42%或95%程度）的温石棉一起培养时，与未处理的温石棉相比，观察到死亡率有统计学上的显著降低。当V79细胞与酸浸或42%疏水改性温石棉一起培养时，即使将人类肺泡巨噬细胞作为激活源，也未观察到诱变活性。与未处理的温石棉相比，95%疏水改性和酸浸的温石棉对苯并[a]芘的结合也有所减少。