Mabuchi I
J Biochem. 1981 Apr;89(4):1341-4.
A protein which is capable of depolymerizing F-actin was purified from an extract of unfertilized starfish eggs by the use of DEAE-cellulose column chromatography and hydroxylapatite column chromatography. This protein has an apparent molecular weight of 17,000. It inhibited the extent of actin polymerization as well as depolymerizing F-actin rapidly. It was shown that this protein reacts with actin at a molar ratio of 1 : 1. The properties of this protein were compared with those of profilin from mammalian tissues.
通过使用DEAE-纤维素柱色谱法和羟基磷灰石柱色谱法，从未受精的海星卵提取物中纯化出一种能够使F-肌动蛋白解聚的蛋白质。这种蛋白质的表观分子量为17,000。它既能抑制肌动蛋白的聚合程度，又能快速使F-肌动蛋白解聚。结果表明，这种蛋白质与肌动蛋白的反应摩尔比为1:1。将这种蛋白质的特性与来自哺乳动物组织的前纤维蛋白的特性进行了比较。
