一种分离脑肌动蛋白的简单方法。
A simple procedure for the isolation of brain actin.
作者信息
Ruscha M F, Himes R H
出版信息
Prep Biochem. 1981;11(3):351-60. doi: 10.1080/00327488108061774.
PMID:6894794
Abstract
Actin has been isolated from bovine brain by forming an actin-containing gel from acetone powders. Solubilization of the gel and chromatography on Sephadex G150 produces actin in good yield. Acetone powders of crude pellets of brain homogenates obtained in the isolation of tubulin produces about 80% of the actin isolated from acetone powder of whole brain. The procedure therefore can be used to isolate both proteins from the same brain.
摘要
通过用丙酮粉形成含肌动蛋白的凝胶，已从牛脑中分离出肌动蛋白。凝胶的溶解和在葡聚糖凝胶G150上的色谱分离可高产率地产生肌动蛋白。在分离微管蛋白过程中获得的脑匀浆粗沉淀的丙酮粉所产生的肌动蛋白，约为从全脑丙酮粉中分离出的肌动蛋白的80%。因此，该方法可用于从同一大脑中分离这两种蛋白质。
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