Neurohr K J, Lacelle N, Mantsch H H, Smith I C
Biophys J. 1980 Dec;32(3):931-8. doi: 10.1016/S0006-3495(80)85027-2.
Deuterium nuclear magnetic resonance is used to delineate the molecular dynamics of sugars bound to a lectin. 2H spin-spin relaxation times (from linewidth measurements) and reorientational correlation times are determined for N-acetylglucosamine specifically-labeled with 2H in the N-acetyl group and at carbon-3 of the pyranoside ring, in the presence and absence of wheat germ agglutinin. The correlation time for the 2H-label of N-acetylglucosamine-3-2H in the bound state is the same as that of the protein (3 X 10(-8)S), indicating that the six-membered ring has negligible motional freedom relative to the protein. The correlation time for the C2H3 group of N-acetyl-2H3-glucosamine (1.7 X 10(-9)S) shows that the N-acetyl side chain is also immobilized in the binding site, the only motion available being rotation of the C2H3 group about its threefold axis.
氘核磁共振用于描绘与凝集素结合的糖的分子动力学。在存在和不存在麦胚凝集素的情况下，测定了在N - 乙酰基和吡喃糖苷环的碳 - 3处用2H特异性标记的N - 乙酰葡糖胺的2H自旋 - 自旋弛豫时间（根据线宽测量）和重取向相关时间。结合状态下N - 乙酰葡糖胺 - 3 - 2H的2H标记的相关时间与蛋白质的相关时间相同（3×10^(-8)秒），表明相对于蛋白质，六元环的运动自由度可忽略不计。N - 乙酰 - 2H3 - 葡糖胺的C2H3基团的相关时间（1.7×10^(-9)秒）表明N - 乙酰侧链在结合位点也被固定，唯一可用的运动是C2H3基团绕其三重轴的旋转。
